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991.
In this paper we will review the results obtained during the last 2 years with the aponeurotic galea and subgalea for vermillion lip augmentation. The survey was carried out on 42 patients who displayed either an absence of or reduction in the vermillion of one or both lips or a senile lip. In all cases the surgical procedure introduced aponeurotic galea and subgalea in the space found between the orbicular lip muscle and the vestibular mucus, just behind the vermillion. All the operations were performed under local anaesthetic. The size of the aponeurotic galea fragment removed varied in length between 10 and 12 cms. and in width between 1 and 2 cms., using the following parameters for its dimensions: the previous volume of the lips, and the distance between the two buccal commissures when in the “smile position.” 相似文献
992.
993.
994.
995.
Jose Carlos de Fraga João Pippi Salle Mauro Boher Carlos R. Antunes 《Pediatric surgery international》1995,10(5-6):376-378
Laryngotracheoesophageal cleft is an uncommon disease that is difficult to diagnose and treat. Repair of the cleft depends on length and localization of the defect as well as the associated anomalies. A successful repair of a type II cleft is reported in this paper. An anterior split of the larynx and trachea was used and provided excellent exposure and safe repair without injury to the neurovascular structures. This is the best approach and should be used to correct all type II defects. 相似文献
996.
Corticosteroids reduce regenerative repair of epithelium in experimental gastric ulcers. 总被引:2,自引:0,他引:2 下载免费PDF全文
The association between corticosteroid treatment and gastric ulcer healing is controversial. The effects of corticosteroids on experimental ulcer healing in the rat were studied and the effect of coadministration of a prostaglandin E1 analogue was determined. Forty male adult rats were divided into five groups and pretreated for 10 days as follows: (a) control, (b) prednisolone (10 mg/kg), (c) prednisolone and misoprostol (300 micrograms/kg), (d) misoprostol, and (e) indomethacin (2 mg/kg) Gastric ulcer was induced by applying a cryoprobe to the serosal surface of the stomach. Healing was assessed by determining the ulcer size at three and six days. Mucosal regenerative activity at the ulcer edge was assessed by quantitating DNA synthesis in cells by immunohistochemical techniques using monoclonal antibodies to detect expression of proliferating cell nuclear antigen (PCNA) and incorporated 5-bromo-5-iododeoxyuridine (BrdU). Compared with control rats, the rate of healing and the mucosal regenerative activity were significantly reduced in rats treated with prednisolone and indomethacin (p < 0.05). Coadministration of misoprostol and corticosteroids reversed the delay in healing and the reduction in mucosal regeneration induced by corticosteroids alone. With misoprostol alone, the ulcer size and the number of epithelial cells that actively synthesised DNA did not differ from control animals. A comparison between the two immunohistochemical markers of cell proliferation showed a highly significant correlation between the two techniques (r = 0.64, p < 0.005), indicating that the simpler PCNA technique should prove valuable in assessing regeneration in experimental ulcer disease. 相似文献
997.
998.
Expression and characterization of the eaeA gene product of Escherichia coli serotype O157:H7. 总被引:7,自引:6,他引:1 下载免费PDF全文
M Louie J C de Azavedo M Y Handelsman C G Clark B Ally M Dytoc P Sherman J Brunton 《Infection and immunity》1993,61(10):4085-4092
In enteropathogenic Escherichia coli, the eaeA gene produces a 94-kDa outer membrane protein called intimin which has been shown to be necessary but not sufficient to produce the attaching-and-effacing lesion. The purpose of this study was to characterize the intimin specified by the eaeA allele of the enterohemorrhagic E. coli (EHEC) serotype O157:H7 strain CL8 and to determine its role in adherence. The carboxyl-terminal 266 amino acids of the CL8 intimin were expressed as a protein fusion with glutathione S-transferase, which was used to raise antiserum in rabbits. The antiserum reacted in Western immunoblots with a 97-kDa outer membrane protein of EHEC strains of serogroups O5, O26, O111, and O157 and enteropathogenic E. coli strains of serogroups O55 and O127. Surface labelling of CL8 with 125I showed that intimin was surface exposed. An eaeA insertional inactivation mutant of CL8 was produced and was designated CL8-KO1. Total adherence of CL8-KO1 to HEp-2 cells was not significantly different from that of CL8, but CL8-KO1 gave a negative result in the fluorescent actin staining test. The eaeA gene expressed alone in E. coli HB101 also gave a negative fluorescent actin staining test result. The eaeA gene of CL8 was able to complement the eaeA deletion mutation in CVD206. We conclude that the product of the EHEC eaeA gene is a 97-kDa surface-exposed protein and propose that it be designated intiminO157. Sherman et al. described a 94-kDa outer membrane protein which played an important role in adherence of E. coli O157:H7 (Infect. Immun. 59:890-899, 1991). Western immunoblotting and indirect fluorescent antibody studies showed that the protein described by Sherman et al. is not intimin. 相似文献
999.
Dermatitis in bulb growers 总被引:2,自引:0,他引:2
Derk P. Bruynzeel Edith M. de Boer Elly J. Brouwer Frederik A. de Wolff Peter de Haan 《Contact dermatitis》1993,29(1):11-15
A damaged skin forms a health hazard in flower-bulb growers as it enables higher permeation rates For pesticides than normal skin. Therefore, an investigation was performed into the skin condition of 103 bulb growers and 49 controls. Contact dermatitis of the hands was of the same order (11 and 10%) in both groups. However, minor signs of dermatitis were seen more often in bulb growers (30 versus 8%, p <0.05). Most growers had contact with narcissus sap during the investigation. This irritant sap, as well as many other skin contacts with irritants such as hyacinth dust and pesticides, seemed to be responsible for many skin complaints. Contact serialization was suspected in 19 growers and 3 controls. Patch tests showed that contact sensitization existed to pesticides in probably 10, and to flower-bulb extracts in 4 growers. Reactions to propachlor were not regarded as very reliable as the test concentration seemed to be marginally irritant. There were only a few allergic reactions to narcissus (3) and tulip (2) and none to hyacinth. This investigation showed that minor irritant contact dermatitis was frequent in bulb growers, and indicated that contact sensitization to pesticides and bulbs seemed to be a less frequent but important cause of dermatitis. 相似文献
1000.
de Boer A; Schroder C; Reddingius R; Willems H; Monnens L 《Nephrology, dialysis, transplantation》1998,13(9):2348-2350
Background: The passage of proteins across the
glomerular filtration barrier is mainly determined by the size of the
protein. In nephrotic syndrome (NS) the glomerular permselectivity is
affected, causing proteinuria. Some authors suggest the existence of a
generalized basement membrane defect. The permeability characteristics of
the peritoneal basement membrane in children with NS are not known.
Methods: The transperitoneal transport of proteins
with a different molecular weight ({beta}2-microglobulin MW 11 800 D,
albumin MW 69 000 D, IgG MW 160 000 D, and &agr;2-macroglobulin MW 820
000 D) was studied in a study group (group A) consisting of six stable
nephrotic children (three with glomerulosclerosis and three with congenital
nephrotic syndrome, one of them with mesangial sclerosis) and compared to a
control group (group B) consisting of eight stable children on peritoneal
dialysis. After a dwell of 6 h with Dianeal 1.36% dialysate and serum
samples were collected. For each patient the dialysate to plasma (D/P)
ratio of the four proteins were calculated. The D/P ratios of the nephrotic
patients in group A were compared to the D/P ratios of the patients in the
control group B. Data were expressed as mean ±SD.
Results: The values for the D/P ratios (in percentage)
of {beta}2-microglobulin, albumin, IgG and &agr;2-macroglobulin in
group A were 19.6±9.9, 2.7±1.7, 1.6±0.9,
and 0.5±0.4, compared to 24.9±10.2,
4.0±2.3, 2.2±1.2, and 0.7±0.3 in the
control group B. The ratios were plotted against MW on a double logarithmic
scale. In all patients a linear relationship between molecular weight and
D/P ratio of the proteins was obtained. The D/P ratios of the study group
did no differ significantly from the control group.
Conclusion: We conclude that the size selectivity of
the capillary permeability is not affected in the peritoneal membrane in
children with NS due to glomerulosclerosis and congenital nephrotic
syndrome. 相似文献